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Bradykinin and histamine‐induced cytosolic calcium increase in capillary endothelial cells of bovine adrenal medulla
Author(s) -
Vinet Raúl,
Cortés Magdalena P.,
Álvarez Rocío,
Delpiano Marco A.
Publication year - 2014
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10300
Subject(s) - thapsigargin , histamine , bradykinin , medicine , endocrinology , chemistry , bradykinin receptor , serca , cyclopiazonic acid , calcium , receptor , biology , biochemistry , atpase , enzyme
We have assessed the effect of bradykinin and histamine on the cytosolic free calcium concentration ([Ca 2+ ] i ) of bovine adrenal medulla capillary endothelial cells (BAMCECs). To measure [Ca 2+ ] i changes in BAMCECs the intracellular fluorescent probe, fluo‐3 AM, was used. Bradykinin (3 µM) produced a transient monophasic increase in [Ca 2+ ] i , which was depressed by B1650 (0.1 µM), a B2‐bradykinin receptor antagonist (D‐Arg‐[Hyp 3 , Thi 5,8 , D‐Phe 7 ]‐Bradykinin). Similarly, increase in [Ca 2+ ] i induced by histamine was also depressed by tripolidine (0.1 µM), an H1‐histamine receptor antagonist. [Ca 2+ ] i increase induced by both agonists was unaffected in the absence of extracellular Ca 2+ or presence of antagonists of voltage operated Ca 2+ channels (VOCCs). Thapsigargin (1 µM) did not abolish the increase of [Ca 2+ ] i produced by bradykinin, but abolished that of histamine. In contrast, caffeine (100 µM), abolished the [Ca 2+ ] i response induced by bradykinin (3 µM), but did not affect the [Ca 2+ ] i increase induced by histamine (100 µM). The results indicate the presence of B2 bradykinin‐ and H1 histamine‐receptors in BAMCECs. Liberation of Ca 2+ induced by both agonists occurs through 2 different intracellular mechanisms. While bradykinin activates a sarco(endo) plasmic reticulum (SER) containing a SER Ca 2+ ‐ATPase (SERCA) thapsigargin‐insensitive, histamine activates a SER containing a SERCA thapsigargin‐sensitive. We suggest that the increase in [Ca 2+ ] i induced by bradykinin and histamine could be of physiological relevance, modulating adrenal gland microcirculation.

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