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Long‐term exposure of 3T3 fibroblast cells to endocrine disruptors alters sensitivity to oxidative injury
Author(s) -
Nishimura Yuka,
Nakai Yasuyoshi,
Tanaka Aiko,
Nagao Tetsuji,
Fukushima Nobuyuki
Publication year - 2014
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10269
Subject(s) - gper , oxidative stress , estrogen receptor , western blot , nonylphenol , receptor , chemistry , downregulation and upregulation , endocrinology , medicine , biology , biochemistry , cancer , breast cancer , gene , environmental chemistry
When Swiss 3T3 fibroblasts were exposed to bisphenol A (BPA) or nonylphenol (NP) within a range of 0.1–100 nM for 30–45 days, increased resistance to oxidative injury was found. Western blot analysis indicated concomitant increased expression of bcl‐2 protein and reduced histone methylation levels in cells after BPA or NP exposure. Using a heterologous expression system, both chemicals could stimulate G protein‐coupled receptor 30 (GPR30), a transmembrane estrogen receptor predominantly expressed in 3T3 cells, at lower concentrations, which gave increased survival. Taken together, these results suggest that BPA or NP exposure might cause alterations in cellular activity against oxidative stress, possibly through GPR30.