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Focal adhesion kinase (FAK) siRNA inhibits human hypertrophic scar by suppressing integrin α, TGF‐β and α‐SMA
Author(s) -
Chen Rui,
Zhang Zhiliang,
Xue Zhujia,
Wang Lin,
Fu Mingang,
Lu Yi,
Bai Ling,
Zhang Dongqing,
Fan Zhihong
Publication year - 2014
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10265
Subject(s) - focal adhesion , transfection , integrin , microbiology and biotechnology , small interfering rna , chemistry , signal transduction , transforming growth factor , biology , cancer research , cell , biochemistry , gene
The effect of focal adhesion kinase (FAK) on suppressing scarring and the potential molecular mechanism underlying it has been investigated. Ten samples of human hypertrophic scars (HS) tissue cultured in vitro were transfected with FAK siRNA mediated by liposome. Quantitative real‐time PCR was used to detect the expression of integrin α, transforming growth factor‐β (TGF‐β), FAK and α‐smooth muscle actin (α‐SMA) after transfection. MTT assay was used as a measure of fibroblast proliferation. Flow cytometry and 3 H‐proincorporation technique gave measurements of the cell cycle and the quantity of collagen synthesis, respectively. Expression of FAK was effectively blocked, accompanied by decreasing expression of integrin α, TGF‐β and α‐SMA in hypertrophic scars fibroblast (HSFB) cells. One to 4 h after transfection with FAK siRNA, proliferation of HSFB cells was strongly inhibited ( P < 0.01), reaching a maximum at 48 h. The proportion of G 1 cells was higher and the proportion of the S and G 2 cells lower after transfection. The amount of collagen synthesis in HSFB cells decreased when HSFB cells were transfected for 48 h. RNA interference targeting the FAK gene can block the two abnormal signal transduction pathways mediated by the integrin and TGF‐β receptors that are responsible for hyperplasia and contracture of the scar, making FAK iRNA therapy a potentially effective approach in HS treatment.