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Hedgehog signalling is dispensable in the proliferation of stem cells from human exfoliated deciduous teeth
Author(s) -
Ejeian Fatemeh,
Baharvand Hossein,
NasrEsfahani Mohammad Hossein
Publication year - 2014
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10227
Subject(s) - sonic hedgehog , biology , progenitor cell , hedgehog signaling pathway , mesenchymal stem cell , stem cell , microbiology and biotechnology , hedgehog , cell growth , cell cycle , population , cell , signal transduction , genetics , medicine , environmental health
The hedgehog (Hh) signalling pathway is one of the key regulators in development with a dual role in cell fate specification, proliferation, and survival on different target cells. We have investigated the effect of recombinant sonic hedgehog (r‐SHH) on extracted multipotent stem cells from human exfoliated deciduous teeth (SHED), which represent a potential stem cell population for therapeutic applications. Cell proliferation and cycle assays shown that r‐SHH did not have a distinctive effect on cell cycle progression, nor did it increase cell number over a wide range of concentrations. Quantitative polymerase chain reaction (Q‐PCR) also suggests that r‐SHH treatment has no demonstrable influence on expression of proliferative genes ( CCNE1 and KI67 ); in contrast, the anti‐proliferative gene ( CDKN1A ) is overexpressed in response to SHH. Our findings have suggested the possibility that SHEDs demonstrate a different potential from human bone marrow mesenchymal stem cells (h‐BMSCs) and dorsal neural progenitor in response to growth factors such as SHH.