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Bone formation in adipose‐derived stem cells isolated from elderly patients with osteoporosis: a preliminary study
Author(s) -
Jiang Ming,
Wang Xin,
Liu Hongzhi,
Zhou Lugang,
Jiang Tingshu,
Zhou Huihui,
Hao Wei
Publication year - 2014
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10182
Subject(s) - bone sialoprotein , alkaline phosphatase , osteocalcin , adipose tissue , chemistry , osteoporosis , fibroblast , pathology , medicine , in vitro , biochemistry , enzyme
We have explored the osteogenic potency of adipose‐derived stem cells from osteoporotic patients (opASCs). opASCs were osteogenically induced in vitro with collagen I hydrogel or in culture plate. Detection of alkaline phosphatase (ALPase) and cell mineralisation, and quantitative RT‐PCR of collagen I, osteocalcin and bone sialoprotein were undertaken. Proliferation and morphology studies were also performed. After 14 days, opASCs‐collagen I hydrogel composite was implanted into nude mice for 4 weeks prior to radiographic and histological analysis. Staining of ALPase and cell mineralisation was strongly positive in opASCs. Fibroblast‐like opASCs induced with collagen I hydrogel were evenly distributed and proliferated at a higher rate than in culture plates, showing similar growth curves for both genders. Expression of ALPase activity, cell mineralisation and osteogenic specific genes were higher in opASCs with collagen I hydrogel (male samples had better osteogenicity than female samples) than in culture plates. After implantation for 4 weeks, radiopaque area signifying new bone tissue was observed in opASCs‐collagen I hydrogel composite, with no donor gender differences. Thus opASCs with collagen I hydrogel have adequate osteogenic potency and offer new possibilities for osteoporosis‐related bone tissue engineering in male and female patients.