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The lectin of Dolichos biflorus agglutinin recognises glycan epitopes on the surface of a subset of cardiac progenitor cells
Author(s) -
Chen Zhanfeng,
Wang Man,
Xiang Qiang,
Sun Zhenliang,
Zhang Rong
Publication year - 2013
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10162
Subject(s) - lectin , epitope , flow cytometry , progenitor cell , biology , agglutinin , microbiology and biotechnology , immunology , chemistry , stem cell , antibody
The discovery of adult cardiac progenitor cells (CPCs) provides a promising way for treating heart disease; however, their surface characteristics that play a critical role in regulating their maintenance, self‐renewal, migration, and differentiation have not been fully investigated. One subpopulation of Dolichos biflorus agglutinin (DBA)‐positive cells was identified in the heart of adult mice. Flow cytometry showed that 3.7% of heart cells could be labeled by FITC conjugated DBA. BrdU pulse‐chase showed that 55–75% of DBA + cells were CPCs. Evidences from 5‐FU‐induced myelosuppression along with BrdU pulse‐chasing suggests that DBA‐positive cells are proliferative. Furthermore, DBA positive cells display a cologenic appearance in vivo. Our findings suggest that DBA‐positive cells in the heart of adult mouse contained a subset of CPCs, and DBA reactivity is one novel surface characteristic on CPCs.