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Potential of co‐culture of nucleus pulposus mesenchymal stem cells and nucleus pulposus cells in hyperosmotic microenvironment for intervertebral disc regeneration
Author(s) -
Tao YiQing,
Liang ChengZhen,
Li Hao,
Zhang YuJie,
Li FangCai,
Chen Gang,
Chen QiXin
Publication year - 2013
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10110
Subject(s) - aggrecan , osmotic concentration , microbiology and biotechnology , viability assay , intervertebral disc , nucleus , mesenchymal stem cell , cell growth , stem cell , regeneration (biology) , chemistry , cell , biology , anatomy , biochemistry , pathology , medicine , alternative medicine , osteoarthritis , articular cartilage
Nucleus pulposus mesenchymal stem cells (NPMSCs) are a potential cell source for intervertebral disc (IVD) regeneration, but little is known about their response to IVD‐like high osmolarity (400 mOsm). This study was to investigate the viability, proliferation and protein biosynthesis of nucleus pulposus cells (NPCs), NPMSCs and co‐cultured NPMSCs‐NPCs under IVD‐like high osmolarity conditions. NPCs and NPMSCs were isolated and cultured under standard and IVD‐like high osmolarity conditions for 1 or 2 weeks. Cell viability was measured by annexin V‐FITC and PI staining, and cell proliferation measured by MTT assay. The expression of SOX‐9, aggrecan and collagen‐II was measured by RT‐PCR and Western blot analyses. IVD‐like high osmolarity condition slightly inhibited cell viability and decreased the expression of SOX‐9, aggrecan and collagen‐II at the mRNA and protein levels in all groups compared with standard condition. NPMSCs could tolerate IVD‐like high osmolarity, and NPCs‐NPMSCs co‐culture increased cell proliferation and the expression of SOX‐9, aggrecan and collagen‐II under both culture conditions, suggesting that co‐culture of NPMSCs‐NPCs has potential application for IVD regeneration.