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Effect of bixin and norbixin on the expression of cytochrome P450 in Hep G 2 cell line
Author(s) -
Matuo Míriam Cristina Sakuragui,
de Oliveira Takamoto Rafael Teruiti,
Kikuchi Irene Satiko,
de Jesus Andreoli Pinto Terezinha
Publication year - 2013
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10108
Subject(s) - inducer , cyp1a2 , cytochrome p450 , chemistry , biochemistry , cyp2b6 , enzyme , cell culture , gene expression , gene , biology , genetics
Bixin and norbixin are the main components of annatto, which is extracted from Bixa orellana and largely used as natural colorant in the food and pharmaceutical industries. Annatto can enhance CYP1A and CYP2B activity in rats; however, the inducer effect has not been investigated in human cell lines. In this study, the ability of bixin and norbixin to induce the cytochrome P450 (CYP) enzymes was assessed in HepG2 human hepatoma cell line. HepG2 cells were treated with bixin and norbixin, and the expression of the CYP genes quantified by real‐time reverse transcription polymerase chain reaction (RT‐PCR). Expression of CYP1A1 and CYP1A2 was significantly increased by bixin treatment, while CYP2B6, 2C9, 2E1 and 3A4 were unaffected. Cells were treated with norbixin showed no inducer effect. The results suggest that the inducer potential of annatto is attributed to bixin, but not to norbixin, despite their similarities in molecular structure.