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Downregulation of mi RNA ‐128 sensitises breast cancer cell to chemodrugs by targeting Bax
Author(s) -
Ji Shufeng,
Shao Guoli,
Lv Xiaobin,
Liu Yuan,
Fan Yingfang,
Wu Aiguo,
Hu Haiyan
Publication year - 2013
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10100
Subject(s) - transfection , downregulation and upregulation , microrna , apoptosis , cancer research , breast cancer , cell culture , cancer , oligonucleotide , cell , microbiology and biotechnology , chemistry , biology , gene , biochemistry , genetics
miR‐128 is more highly expressed in drug‐resistant breast cancer samples than in drug‐sensitive samples. We have confirmed that Bax is the target of miR‐128 by negative post‐transcriptional regulation. miR‐128 and Bax were detected in the breast cancer cell line, MDA‐MB‐231, which was then transfected with miR‐128 MIMIC (precursor of miR‐128) or AMO (antisense‐miR‐128 oligonucleotides). After transfection, the chemosensitivity of MDA‐MB‐231 cell was up‐regulated with increasing of Bax and inhibition of miR‐128.