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Identification of radiation‐sensitive expressed genes in the ICR and AKR/J mouse thymus
Author(s) -
Bong Jin Jong,
Kang Yu Mi,
Shin Suk Chul,
Choi Seung Jin,
Lee Kyung Mi,
Kim Hee Sun
Publication year - 2013
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10065
Subject(s) - ionizing radiation , tunel assay , apoptosis , microbiology and biotechnology , irradiation , terminal deoxynucleotidyl transferase , gene , biology , chemistry , biochemistry , physics , nuclear physics
We have investigated radiation‐sensitive expressed genes (EGs), their signal pathways, and the effects of ionizing radiation in the thymus of ICR and AKR/J mice. Whole‐body and relative thymus weights were taken and microarray analyses were done on the thymuses of high‐dose‐rate (HDR, 137 Cs, 0.8 Gy/min, a single dose of 4.5 Gy) and low‐dose‐rate (LDR, 137 Cs, 0.7 mGy/h, a cumulative dose of 1.7 Gy) irradiated ICR and AKR/J mice. Gene expression patterns were validated by quantitative polymerase chain reaction (qPCR). The effect of ionizing radiation on thymus cell apoptosis was measured terminal deoxynucleotidyl‐transferase‐mediated dUTP‐end labeling (TUNEL). LDR‐irradiation increased the mean whole‐body weight, but decreased the relative thymus weight of AKR/J mice. Radiation‐sensitive EGs were found by comparing HDR‐ and LDR‐irradiated ICR and AKR/J mice. qPCR analysis showed that 12 EGs had dose and dose‐rate dependent expression patterns. Gene‐network analysis indicated that Ighg, Igh‐VJ558, Defb6, Reg3g, and Saa2 may be involved in the immune response, leukocyte migration, and apoptosis. Our data suggest that expression of the HDR (Glut1, Glut4, and PKLR) and LDR radiation‐response genes (Ighg and Igh‐VJ558) can be dose or dose‐rate dependent. There was an increased number of apoptotic cells in HDR‐irradiated ICR mice and LDR‐irradiated AKR/J mice. Thus, changes of the mean whole‐body weight and relative thymus weight, EGs, signal pathways, and the effects of ionizing radiation on the thymus of ICR and AKR/J mice are described.