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Role of altholactone in inducing type II apoptosis signalling pathway and expression of cancer‐related genes in cervical carcinoma HeLa cell line
Author(s) -
UthaisangTanechpongtamb Wanlaya,
Sriyabhaya Promjit,
Wilairat Prapon
Publication year - 2013
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10059
Subject(s) - hela , apoptosis , cytotoxicity , cytochrome c , biology , mtt assay , microbiology and biotechnology , cytotoxic t cell , cell culture , cancer cell , cancer research , cancer , biochemistry , in vitro , genetics
Goniothalamus species (Annonaceae) is a shrub that grows in the rainforest of tropical Asia. Several compounds have been isolated and exhibit the potential use for cancer treatment. In this work, altholactone isolated from Goniothalamus macrophyllus was investigated for its cytotoxicity, apoptosis signalling and the expression of cancer‐related genes in the cervical carcinoma HeLa cells. Cytotoxicity was evaluated by MTT assay. Apoptotic characteristics were evaluated by morphological studies. Caspase‐3 activity was detected using a fluorogenic substrate. Cytochrome c release from mitochondria and protein Bid were determined by Western blotting and cancer‐related genes expression by RT‐PCR. The results demonstrated that altholactone was cytotoxic to HeLa (IC 50 = 9.6 μg/mL), and apoptotic cell death was manifested by appearance of chromatin condensation and caspase‐3 activation, which was inhibited by specific inhibitors of both caspase‐8 and ‐9. Release into the cytosol of cytochrome and cleavage of Bid occurred. Altholactone also caused a decrease in bcl‐2 and an increase in p53 expression. These unique properties of altholactone suggest a potential for cancer chemotherapy.