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Aurora B kinase is required for cytokinesis through effecting spindle structure
Author(s) -
Wu Qiang,
Zhang Wei,
Mu Tianhao,
Song Tianqiang,
Li Dengwen
Publication year - 2013
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10057
Subject(s) - aurora b kinase , cytokinesis , microbiology and biotechnology , midbody , mitosis , kinetochore , spindle pole body , spindle apparatus , spindle checkpoint , microtubule , multipolar spindles , advanced spaceborne thermal emission and reflection radiometer , polo like kinase , biology , aurora inhibitor , cell division , cell cycle , physics , cell , genetics , chromosome , satellite , gene , astronomy
The spindle, composed of microtubules and associated proteins, serves as major machinery for cell division. Aurora B is a chromosome passenger protein that has important functions in mitosis. Its dynamic distribution at mitosis goes along with spindle structure and dynamics. We used the siRNA technique to knockdown protein expression and immunofluorescence technique to follow Aurora B during mitosis. Aurora B regulates microtubule plus ends as mitosis progresses, including both kinetochore and polar microtubules. Interactions between Aurora B and polar microtubule plus ends lead to failure in cytokinesis and abnormal midbody structure. We think Aurora B may not only play a role as kinase, but regulate microtubule plus ends.