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Enhanced maintenance of rat islets of Langerhans on laminin‐coated electrospun nanofibrillar matrix in vitro
Author(s) -
Sojoodi Mozhdeh,
Farrokhi Ali,
Moradmand Azadeh,
Baharvand Hossein
Publication year - 2013
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10045
Subject(s) - islet , laminin , fibronectin , extracellular matrix , microbiology and biotechnology , in vitro , pancreatic islets , transplantation , chemistry , biology , insulin , medicine , endocrinology , biochemistry
Understanding the extracellular matrix (ECM) effect on pancreatic β cells is critical to optimise the derivation of functional β cells for transplantation and understand mechanisms that control islet neogenesis and glucose homeostasis. We assessed the effect of natural ECMs [collagen I, collagen IV, laminin and fibronectin (FN)] on rat islets of Langerhans' morphology, adhesion, viability, functionality and islet specific genes expression after 7 days in vitro culture. However, we could not detect a significant difference on the other parameters in these ECMs and islets interaction. To examine islets interactions, we used a synthetic three‐dimensional surface composed of electrospun polyamide nanofibres. Laminin‐coated nanofibrillar surfaces, but not laminin or nanosurface alone, induced comparable expression of the Ins1 and Ins2 genes in adult β cells. Using a glucose challenge test, a marked response of insulin secretion by islets occurred that were cultured on laminin‐coated nanofibrillar surfaces. We contend that the reestablishment of cellular interactions by the combination of nanomaterials and natural ECMs can be useful in maintaining in vitro islet functions.