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Cover Feature: N 3 ‐Kethoxal‐Based Bioorthogonal Intracellular RNA Labeling (ChemBioChem 9/2021)
Author(s) -
Huang Jinguo,
Zhao Ruiqi,
Mo Jing,
Wang Fang,
Weng Xiaocheng,
Zhou Xiang
Publication year - 2021
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202100175
Subject(s) - bioorthogonal chemistry , rna , intracellular , covalent bond , chemistry , cover (algebra) , combinatorial chemistry , reagent , riboswitch , aptamer , biophysics , biochemistry , computational biology , click chemistry , non coding rna , microbiology and biotechnology , biology , organic chemistry , mechanical engineering , engineering , gene
The make‐up of RNA : This cover feature describes a new strategy to modify RNA combined with N 3 ‐kethoxal labeling and an intracellular biorthogonal reaction. N 3 ‐kethoxal with the advantages of small size, low toxicity, excellent membrane‐permeable, and high reactivity, serves to bring an azido group to the RNA molecules. Next the cell‐permeable dibenzocyclooctyne (DBCO) reagent reacts with azido group to link covalently to the RNA strand, which can be extended to “make‐up” RNA inside the cell for imaging or other functionalization. More information can be found in the Communication by X. Weng, X. Zhou, et al.