Premium
Flexible Assembly of Engineered Tetrahymena Ribozymes Forming Polygonal RNA Nanostructures with Catalytic Ability
Author(s) -
Mori Yuki,
Oi Hiroki,
Suzuki Yuki,
Hidaka Kumi,
Sugiyama Hiroshi,
Endo Masayuki,
Matsumura Shigeyoshi,
Ikawa Yoshiya
Publication year - 2021
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202100109
Subject(s) - ribozyme , tetrahymena , nucleic acid , rna , chemistry , vs ribozyme , oligomer , hairpin ribozyme , nanotechnology , biophysics , combinatorial chemistry , biochemistry , materials science , biology , organic chemistry , gene
Ribozymes with modular architecture constitute an attractive class of structural platforms for design and construction of nucleic acid nanostructures with biological functions. Through modular engineering of the Tetrahymena ribozyme, we have designed unit RNAs (L‐RNAs), assembly of which formed ribozyme‐based closed trimers and closed tetramers. Their catalytic activity was dependent on oligomer formation. In this study, the structural variety of L‐RNA oligomers was extended by tuning their structural elements, yielding closed pentamers and closed hexamers. Their assembly properties were analyzed by electrophoretic mobility shift assay (EMSA) and atomic force microscopy (AFM).