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Application of Neuron‐Selective Fluorescent Probe, NeuA, To Identify Mouse Retinal Degeneration
Author(s) -
Park SungJin,
Woon Queenie Tan Shu,
Er Jun Cheng,
Wong Bernice H.,
Liu Xiao,
Kang NamYoung,
Barathi Veluchamy A.,
Silver David L.,
Chang YoungTae
Publication year - 2021
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202100011
Subject(s) - retinal degeneration , fluorescence , retinal , neuron , degeneration (medical) , retina , neuroscience , chemistry , biophysics , biology , medicine , ophthalmology , biochemistry , physics , optics
The retina is part of the central nerve system (CNS) and has various interneurons and sensory neurons such as photoreceptor cells. Retinitis pigmentosa (RP) is an inherited condition that is characterized by photoreceptor degeneration. Herein, we developed a fluorescent probe‐NeuA‐for detecting retinal neuronal cells and applied NeuA to discriminate between healthy and RP retinas. The staining pattern of NeuA in the retinas of healthy and RP mouse models was examined in vitro , ex vivo and in vivo using confocal microscopy, the fluorescent fundus microscopy and optical coherent tomography (OCT). NeuA strongly stained the outer segment layer of photoreceptor cells and some bipolar cells in the healthy retina, but there was only weak staining in the photoreceptor degenerated retinas. Therefore, NeuA probe can be used as the detecting RP tools in the preclinical conditions.