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Tetrahedral DNA Nanoconjugates for Simultaneous Measurement of Telomerase Activity and miRNA
Author(s) -
Jiang Chenyu,
Meng Fanyu,
Mao Dongsheng,
Tang Yuguo,
Miao Peng
Publication year - 2021
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000784
Subject(s) - nanodot , nanotechnology , conjugated system , nanostructure , colloidal gold , telomerase , nanomaterials , materials science , dna , nanoparticle , fluorescence , biocompatible material , biophysics , chemistry , biology , biochemistry , gene , biomedical engineering , quantum mechanics , medicine , physics , composite material , polymer
In this study, a tetrahedral DNA nanostructure was first self‐assembled; this was then conjugated with gold nanoparticles (AuNPs) and carbon nanodots (CDs). The fabricated nanocomposites allow simultaneous analysis of telomerase activity and miRNA with dual fluorescence channels. By further introducing an iRGD peptide sequence, the nanoconjuates can be conveniently transferred inside living cells for in situ imaging. The analytical performances and anti‐jamming capabilities are excellent. Meanwhile, the materials are highly biocompatible for intracellular applications. Therefore, the proposed biosystem shows great promise as a powerful tool for quantitative analysis of the dual biomarkers. The strategy can also be further exploited as a versatile platform for in situ detection of many other targets for early disease diagnosis.