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Laccases are multi‐copper oxidases that catalyze the oxidation of various electron‐rich substrates with concomitant reduction of molecular oxygen to water. The multi‐copper oxidase/laccase CueO of  Escherichia coli  is responsible for the oxidation of Cu +  to the less harmful Cu 2+  in the periplasm. CueO has a relatively broad substrate spectrum as laccase, and its activity is enhanced by copper excess. The aim of this study was to trigger CueO activity  in vivo  for the use in biocatalysis. The addition of 5 mM CuSO 4  was proven effective in triggering CueO activity at need with minor toxic effects on  E. coli  cells. Cu‐treated  E. coli  cells were able to convert several phenolic compounds to the corresponding dimers. Finally, the endogenous CueO activity was applied to a four‐step cascade, in which coniferyl alcohol was converted to the valuable plant lignan (−)‐matairesinol.

Use of Copper as a Trigger for the in Vivo Activity of E. coli Laccase CueO: A Simple Tool for Biosynthetic Purposes