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N 3 ‐Kethoxal‐Based Bioorthogonal Intracellular RNA Labeling
Author(s) -
Huang Jinguo,
Zhao Ruiqi,
Mo Jing,
Wang Fang,
Weng Xiaocheng,
Zhou Xiang
Publication year - 2021
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000755
Subject(s) - bioorthogonal chemistry , intracellular , rna , click chemistry , chemistry , isotopic labeling , combinatorial chemistry , biochemistry , organic chemistry , gene
There is growing interest in developing intracellular RNA tools. Herein, we describe a strategy for N 3 ‐kethoxal (N 3 K)‐based bioorthogonal intracellular RNA functionalization. With N 3 K labeling followed by an in vivo click reaction with DBCO derivatives, RNA can be modified with fluorescent or phenol groups. This strategy provides a new way of labeling RNA inside cells.
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