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Chemical Labeling of Protein 4′‐Phosphopantetheinylation
Author(s) -
Chen Nan,
Wang Chu
Publication year - 2021
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000747
Subject(s) - biochemistry , nonribosomal peptide , chemical biology , function (biology) , biosynthesis , protein function , chemistry , biology , protein biosynthesis , computational biology , microbiology and biotechnology , gene
Abstract Nature uses a diverse array of protein post‐translational modifications (PTMs) to regulate protein structure, activity, localization, and function. Among them, protein 4′‐phosphopantetheinylation derived from coenzyme A (CoA) is an essential PTM for the biosynthesis of fatty acids, polyketides, and nonribosomal peptides in prokaryotes and eukaryotes. To explore its functions, various chemical probes mimicking the natural structure of 4′‐phosphopantetheinylation have been developed. In this minireview, we summarize these chemical probes and describe their applications in direct and metabolic labeling of proteins in bacterial and mammalian cells.