Premium
Enhancing the Nucleolytic Resistance and Bioactivity of Functional Nucleic Acids by Diverse Nanostructures through in Situ Polymerization‐Induced Self‐assembly
Author(s) -
Yang Lu,
Liang Mingwei,
Cui Cheng,
Li Xiaowei,
Li Long,
Pan Xiaoshu,
Yazd Hoda Safari,
Hong Min,
Lu Jianrong,
Cao Y. Charles,
Tan Weihong
Publication year - 2021
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000712
Subject(s) - polymerization , aptamer , nucleic acid , nuclease , oligonucleotide , combinatorial chemistry , chemistry , biophysics , micelle , nanotechnology , dna , biochemistry , materials science , biology , microbiology and biotechnology , organic chemistry , aqueous solution , polymer
Functional nucleic acids (FNAs) are garnering tremendous interest owing to their high modularity and unique bioactivity. Three‐dimensional FNAs have been developed to overcome the issues of nuclease degradation and limited cell uptake. We have developed a new facile approach to the synthesis of multiple three‐dimensional FNA nanostructures by harnessing photo‐polymerization‐induced self‐assembly. Sgc8 aptamer and CpG oligonucleotide were modified as macro chain‐transfer reagents to mediate in situ polymerization and self‐assembly. Diverse structures, including micelles, rods, and short worms, afford these two FNAs afford these two FNAs with higher nuclease resistance in serum serum, greater cellular uptake efficiency, and increased bioactivity.