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Covalently Mercurated Molecular Beacon for Discriminating the Canonical Nucleobases
Author(s) -
AroHeinilä Asmo,
Lönnberg Tuomas,
Virta Pasi
Publication year - 2021
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000575
Subject(s) - nucleobase , chemistry , oligonucleotide , molecular beacon , selectivity , covalent bond , nucleoside , pyrimidine , stereochemistry , fluorescence , nucleotide , affinities , combinatorial chemistry , dna , biochemistry , organic chemistry , gene , physics , quantum mechanics , catalysis
A highly nucleobase‐discriminating metalated nucleoside analogue, 3‐fluoro‐2‐mercuri‐6‐methylaniline, was incorporated into an oligonucleotide molecular beacon. Fluorescence emission spectra were measured after the addition of four different complementary strands, in which the nucleobase opposite the metalated analogue varies. The fluorescence results showed a clear binding selectivity at room temperature, in the order G>T>C>A. The selectivity is based on the different affinities between the metalated nucleoside analogue and the canonical nucleobases. The synthesized probe is capable of robust discrimination between the two purine as well as the two pyrimidine bases by fluorescence at room temperature, and more sophisticated temperature analysis allows clear separation of every canonical nucleobase. The probe would, hence, be a suitable method for the detection of single nucleotide polymorphisms.