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Regulating CRISPR/Cas9 Function through Conditional Guide RNA Control
Author(s) -
Brown Wes,
Zhou Wenyuan,
Deiters Alexander
Publication year - 2021
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000423
Subject(s) - crispr , cas9 , guide rna , computational biology , genome editing , rna , biology , function (biology) , transcription (linguistics) , oligonucleotide , computer science , gene , genetics , linguistics , philosophy
Conditional control of CRISPR/Cas9 has been developed by using a variety of different approaches, many focusing on manipulation of the Cas9 protein itself. However, more recent strategies for governing CRISPR/Cas9 function are based on guide RNA (gRNA) modifications. They include control of gRNAs by light, small molecules, proteins, and oligonucleotides. These designs have unique advantages compared to other approaches and have allowed precise regulation of gene editing and transcription. Here, we discuss strategies for conditional control of gRNA function and compare effectiveness of these methods.