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Effect of Photocaged Isopropyl β‐ d ‐1‐thiogalactopyranoside Solubility on the Light Responsiveness of LacI‐controlled Expression Systems in Different Bacteria
Author(s) -
Hogenkamp Fabian,
Hilgers Fabienne,
Knapp Andreas,
Klaus Oliver,
Bier Claus,
Binder Dennis,
Jaeger KarlErich,
Drepper Thomas,
Pietruszka Jörg
Publication year - 2021
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000377
Subject(s) - lac repressor , chemistry , bacteria , inducer , lac operon , bacillus subtilis , repressor , gene expression , lipophilicity , biophysics , biochemistry , escherichia coli , microbiology and biotechnology , combinatorial chemistry , gene , biology , genetics
Photolabile protecting groups play a significant role in controlling biological functions and cellular processes in living cells and tissues, as light offers high spatiotemporal control, is non‐invasive as well as easily tuneable. In the recent past, photo‐responsive inducer molecules such as 6‐nitropiperonyl‐caged IPTG (NP‐cIPTG) have been used as optochemical tools for Lac repressor‐controlled microbial expression systems. To further expand the applicability of the versatile optochemical on‐switch, we have investigated whether the modulation of cIPTG water solubility can improve the light responsiveness of appropriate expression systems in bacteria. To this end, we developed two new cIPTG derivatives with different hydrophobicity and demonstrated both an easy applicability for the light‐mediated control of gene expression and a simple transferability of this optochemical toolbox to the biotechnologically relevant bacteria Pseudomonas putida and Bacillus subtilis . Notably, the more water‐soluble cIPTG derivative proved to be particularly suitable for light‐mediated gene expression in these alternative expression hosts.