Premium
An Iodinated DAPI‐Based Reagent for Mass Cytometry
Author(s) -
Rana Rahul,
Chang Qing,
Bassan Jay,
Chow Sue,
Hedley David,
Nitz Mark
Publication year - 2021
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000369
Subject(s) - dapi , mass cytometry , fluorescence , chemistry , reagent , dna , flow cytometry , staining , combinatorial chemistry , biophysics , biochemistry , microbiology and biotechnology , biology , organic chemistry , physics , genetics , quantum mechanics , apoptosis , gene , phenotype
Abstract Multiparametric single‐cell analysis has seen dramatic improvements with the introduction of mass cytometry (MC) and imaging mass cytometry (IMC ™ ). These technologies expanded the number of biomarkers that can be identified simultaneously by using heavy‐isotope‐tagged antibody reagents. Small‐molecule probes bearing heavy isotopes are emerging as additional useful functional reporters of cellular features. Realizing this, we explored the iodination of DAPI to produce a heavy‐atom‐substituted derivative of the commonly used fluorescent DNA stain. Although exhibiting a drastically reduced fluorescence emission profile, I‐DAPI retains strong binding affinity for DNA. I‐DAPI was used to detect cellular DNA in MC and IMC™ assays with comparable efficiency to known Ir‐containing DNA intercalators. This work suggests repurposing well‐known colorimetric stains through simple reactions could be an effective strategy to develop new, functional MC and IMC™ reagents.