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Quantitative Elemental Analysis of a Single Cell by Using Inductively Coupled Plasma‐Mass Spectrometry in Fast Time‐Resolved Analysis Mode
Author(s) -
Tanaka Yuki,
Iida Risako,
Takada Shohei,
Kubota Tetsuo,
Yamanaka Michiko,
Sugiyama Naoki,
Abdelnour Yolande,
Ogra Yasumitsu
Publication year - 2020
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000358
Subject(s) - inductively coupled plasma mass spectrometry , chemistry , mass spectrometry , elemental analysis , inductively coupled plasma , analytical chemistry (journal) , sulfur , chromatography , zinc , plasma , inorganic chemistry , physics , organic chemistry , quantum mechanics
The elemental composition of a single yeast, green alga, or red blood cell (RBC) was precisely determined by using inductively coupled plasma‐mass spectrometry (ICP‐MS) operating in fast time‐resolved analysis (TRA) mode. The technique is known as single‐cell (SC)‐ICP‐MS. Phosphorus, sulfur, magnesium, zinc, and iron were detected in the three types of cell. The elemental composition of yeast and green alga obtained by SC‐ICP‐MS was consistent with results obtained from conventional ICP‐MS measurements following acid digestion of the cells. Slight differences were found in the measured values between SC‐ICP‐MS and the conventional ICP‐MS results for RBC. However, the SC‐ICP‐MS results for S and Fe in RBC were closer to the estimated values for these elements that were calculated from the level of hemoglobin in RBCs. The data suggest that SC‐ICP‐MS is suitable for the analysis of various cell types, namely, fungus, plant, and animal cells.