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Chemical Approaches To Analyzing RNA Structure Transcriptome‐Wide
Author(s) -
England Whitney E.,
Garfio Chely M.,
Spitale Robert C.
Publication year - 2021
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000340
Subject(s) - rna , computational biology , nucleic acid structure , transcriptome , function (biology) , chemical biology , biology , nanotechnology , microbiology and biotechnology , gene expression , genetics , gene , materials science
RNA molecules can fold into complex two‐ and three‐dimensional shapes that are critical for their function. Chemical probes have long been utilized to interrogate RNA structure and are now considered invaluable resources in the goal of relating structure to function. Recently, the power of deep sequencing and careful chemical probe design have merged, permitting researchers to obtain a holistic understanding of how RNA structure can be utilized to control RNA biology transcriptome‐wide. Within this review, we outline the recent advancements in chemical probe design for interrogating RNA structures inside cells and discuss the recent advances in our understanding of RNA biology through the lens of chemical probing.