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First Ring‐Expanded Maytansin Lactone Accessed by a New Mutasynthetic Variant
Author(s) -
Wesemann Friederike,
Heutling Anja,
Wienecke Paul,
Kirschning Andreas
Publication year - 2020
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000336
Subject(s) - mutant , biosynthesis , ring (chemistry) , enzyme , lactone , stereochemistry , chemistry , biology , biochemistry , gene , organic chemistry
A multiblocked mutant strain (ΔAHBA and Δ asm12 , asm21 ) of Actinosynnema pretiosum , the producer of the highly toxic maytansinoid ansamitocin, has been used for the mutasynthetic production of new proansamitocin derivatives. The use of mutant strains that are blocked in the biosynthesis of an early building block as well as in the expression of two tailoring enzymes broadens the scope of chemo‐biosynthetic access to new maytansinoids. Remarkably, a ring‐expanded macrolactone derived from ansamitocin was created for the first time.