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Complete Photochemical Regulation of 8–17 DNAzyme Activity by Using Reversible DNA Photo‐crosslinking
Author(s) -
Watanabe Yasuha,
Fujimoto Kenzo
Publication year - 2020
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000227
Subject(s) - deoxyribozyme , exonuclease , photochemistry , dna , chemistry , substrate (aquarium) , in vivo , biophysics , exonuclease iii , biochemistry , biology , dna polymerase , ecology , microbiology and biotechnology , escherichia coli , gene
Abstract The regulation of DNAzyme activity is an important problem for its in vivo applications. We achieved photochemical regulation of DNAzyme activity by using reversible DNA photo‐crosslinking of 3‐cyanovinylcarbazole ( CNV K). The ODN containing CNV K photo‐crosslinked to a pyrimidine base in the complementary strand after a few seconds of photoirradiation, and its photoadduct was split by photoirradiation of another wavelength. The activity of photo‐crosslinked DNAzyme with CNV K was completely inhibited (OFF state). In contrast, after 312 nm irradiation, DNAzyme activity was recovered upon addition of a substrate strand (ON state). In addition, the photo‐crosslinked DNAzyme is prone to enzymatic digestion by exonuclease. This photochemical OFF to ON switching with reversible DNA photo‐crosslinking was regulated at the desired time and position; therefore, it might be possible to use it for in vivo application.