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Cover Feature: A Trifunctional Linker for Palmitoylation and Peptide and Protein Localization in Biological Membranes (ChemBioChem 9/2020)
Author(s) -
Syga Łukasz,
Vries Reinder H.,
Oosterhout Hugo,
Bartelds Rianne,
Boersma Arnold J.,
Roelfes Gerard,
Poolman Bert
Publication year - 2020
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000217
Subject(s) - palmitoylation , linker , membrane , chemistry , vesicle , peptide , fluorescence , membrane protein , covalent bond , biophysics , green fluorescent protein , biological membrane , peripheral membrane protein , integral membrane protein , biochemistry , biology , cysteine , organic chemistry , enzyme , physics , quantum mechanics , computer science , gene , operating system
The structure of a trifunctional linker is shown. The linker contains a fluorescent dye (green), two palmitoyl moieties (light blue) and is covalently coupled to a protein (schematically shown in dark blue). The tripartite is used to study the effect palmitoylation on the localization of membrane proteins or peptides in giant‐unilamellar vesicles (GUVs) composed of a phase‐separating lipid mixture. Here, the palmitoylated protein is shown to localize to the liquid‐disordered part of the membrane (thinner membrane) as shown by the co‐localization with a specific lipid probe (red). More information can be found in the full paper by G. Roelfes, B. Poolman et al. on page 1320 in Issue 9, 2020 (DOI: 10.1002/cbic.201900655).