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Nonribosomal Peptides Produced by Minimal and Engineered Synthetases with Terminal Reductase Domains
Author(s) -
Tietze Andreas,
Shi YanNi,
Kronenwerth Max,
Bode Helge B.
Publication year - 2020
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000176
Subject(s) - nonribosomal peptide , thioester , reductase , biosynthesis , enzyme , stereochemistry , biochemistry , chemistry , protein engineering , biology
Nonribosomal peptide synthetases (NRPSs) use terminal reductase domains for 2‐electron reduction of the enzyme‐bound thioester releasing the generated peptides as C‐terminal aldehydes. Herein, we reveal the biosynthesis of a pyrazine that originates from an aldehyde‐generating minimal NRPS termed ATRed in entomopathogenic Xenorhabdus indica . Reductase domains were also investigated in terms of NRPS engineering and, although no general applicable approach was deduced, we show that they can indeed be used for the production of similar natural and unnatural pyrazinones.