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The Selectivity of Fosfosal for STAT5b over STAT5a is Mediated by Arg566 in the Linker Domain
Author(s) -
Gräb Julian,
Berg Thorsten
Publication year - 2020
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000111
Subject(s) - linker , chemistry , small molecule , domain (mathematical analysis) , selectivity , computational biology , prodrug , sh2 domain , combinatorial chemistry , biochemistry , biology , phosphorylation , computer science , catalysis , proto oncogene tyrosine protein kinase src , mathematical analysis , mathematics , operating system
Fosfosal is the O‐phosphorylated derivative of salicylic acid, with documented clinical use as a prodrug for the treatment of inflammatory diseases. We recently discovered that fosfosal itself inhibits the protein‐protein interaction domain, the SH2 domain, of the tumor‐related transcription factor STAT5b. Here, we demonstrate that fosfosal is selective for STAT5b over its close homologue STAT5a. This selectivity is mediated by the STAT5b residue Arg566, located in the SH2 domain‐adjacent linker domain. Our data provide further evidence for the role of the STAT linker domain in determining the activity of small molecules against the SH2 domain. We present a refined binding model for fosfosal and STAT5b, which can serve as the basis for the development of fosfosal‐based STAT5b inhibitors.