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Mechanism and Application of the Catalytic Reaction of [NiFe] Hydrogenase: Recent Developments
Author(s) -
Tai Hulin,
Hirota Shun
Publication year - 2020
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000058
Subject(s) - heterolysis , hydrogenase , catalysis , electron transfer , active site , reaction mechanism , chemistry , redox , nanotechnology , combinatorial chemistry , materials science , photochemistry , organic chemistry
Hydrogenases (H 2 ase) catalyze the oxidation of dihydrogen and the reduction of protons with remarkable efficiency, thereby attracting considerable attention in the energy field due to their biotechnological potential. For this simple reaction, [NiFe] H 2 ase has developed a sophisticated but intricate mechanism with the heterolytic cleavage of dihydrogen, where its Ni−Fe active site exhibits various redox states. Recently, new spectroscopic and crystal structure studies of [NiFe] H 2 ases have been reported, providing significant insights into the catalytic reaction mechanism, hydrophobic gas‐access tunnel, proton‐transfer pathway, and electron‐transfer pathway of [NiFe] H 2 ases. In addition, [NiFe] H 2 ases have been shown to play an important role in biofuel cell and solar dihydrogen production. This concept provides an overview of the biocatalytic reaction mechanism and biochemical application of [NiFe] H 2 ases based on the new findings.