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Responsive Fluorophore Aggregation Provides Spectral Contrast for Fluorescence Lifetime Imaging
Author(s) -
Schleyer Kelton A.,
Datko Benjamin D.,
Burnside Brandon,
Cui Chao,
Ma Xiaowei,
Grey John K.,
Cui Lina
Publication year - 2020
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000056
Subject(s) - fluorophore , fluorescence , moiety , chemistry , aggregation induced emission , macromolecule , biophysics , fluorescein , conjugate , fluorescence lifetime imaging microscopy , photochemistry , glutathione , biochemistry , stereochemistry , mathematical analysis , physics , mathematics , quantum mechanics , biology , enzyme
Fluorophores experience altered emission lifetimes when incorporated into and liberated from macromolecules or molecular aggregates; this trend suggests the potential for a fluorescent, responsive probe capable of undergoing self‐assembly and aggregation and consequently altering the lifetime of its fluorescent moiety to provide contrast between the active and inactive probes. We developed a cyanobenzothioazole‐fluorescein conjugate ( 1 ), and spectroscopically examined the lifetime changes caused by its reduction‐induced aggregation in vitro . A decrease in lifetime was observed for compound 1 in a buffered system activated by the biological reducing agent glutathione, thus suggesting a possible approach for designing responsive self‐aggregating lifetime imaging probes.