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Front Cover: An Unintentional Discovery of a Fluorogenic DNA Probe for Ribonuclease I (ChemBioChem 4/2020)
Author(s) -
Chang Dingran,
Chang Thomas,
Salena Bruno,
Li Yingfu
Publication year - 2020
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000038
Subject(s) - ribonuclease , dna , rnase p , cleave , ribonucleotide , nucleotide , chemistry , biochemistry , biology , microbiology and biotechnology , rna , gene
Serendipity : We report the discovery of RFA13‐1, a fluorogenic DNA probe, isolated from a DNA pool (left) against the bacterium Clostridium difficile , which was unintentionally contaminated by another bacterium Klebsiella aerogenes (right). The molecular target of RFA13‐1 has been identified as ribonuclease I from K. aerogenes . We found the selected DNA sequence in RFA13‐1 can assist RNase I (the sharp axe) to efficiently cleave a ribonucleotide embedded in a DNA and shielded by a pair of fluorophore‐labelled and quencher‐labelled nucleotides. More information can be found in the communication by Y. Li et al. on page 464 in Issue 4, 2020 (DOI: 10.1002/cbic.201900455).