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Light‐Driven ATP Regeneration in Diblock/Grafted Hybrid Vesicles
Author(s) -
Kleineberg Christin,
Wölfer Christian,
Abbasnia Amirhossein,
Pischel Dennis,
Bednarz Claudia,
Ivanov Ivan,
Heitkamp Thomas,
Börsch Michael,
Sundmacher Kai,
VidakovićKoch Tanja
Publication year - 2020
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201900774
Subject(s) - bacteriorhodopsin , membrane , vesicle , polymer , biocompatibility , protocell , artificial cell , lipid vesicle , biophysics , liposome , synthetic membrane , chemistry , materials science , nanotechnology , biochemistry , biology , organic chemistry
Light‐driven ATP regeneration systems combining ATP synthase and bacteriorhodopsin have been proposed as an energy supply in the field of synthetic biology. Energy is required to power biochemical reactions within artificially created reaction compartments like protocells, which are typically based on either lipid or polymer membranes. The insertion of membrane proteins into different hybrid membranes is delicate, and studies comparing these systems with liposomes are needed. Here we present a detailed study of membrane protein functionality in different hybrid compartments made of graft polymer PDMS‐g‐PEO and diblock copolymer PBd‐PEO. Activity of more than 90 % in lipid/polymer‐based hybrid vesicles could prove an excellent biocompatibility. A significant enhancement of long‐term stability (80 % remaining activity after 42 days) could be demonstrated in polymer/polymer‐based hybrids.