Premium
Cover Feature: A Quencher‐Free Linear Probe from Serinol Nucleic Acid with a Fluorescent Uracil Analogue (ChemBioChem 1‐2/2020)
Author(s) -
Murayama Keiji,
Asanuma Hiroyuki
Publication year - 2020
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201900761
Subject(s) - uracil , chemistry , nucleic acid , fluorescence , rna , quenching (fluorescence) , monomer , duplex (building) , stereochemistry , förster resonance energy transfer , dna , biophysics , biochemistry , organic chemistry , biology , physics , quantum mechanics , gene , polymer
A quencher‐free linear probe composed of serinol nucleic acid (SNA) with a fluorescent uracil analogue (PeU) can selectively light‐up a target RNA. In the single‐stranded state, a flexible SNA scaffold (yellow strand) facilitates the quenching of monomer emission by aggregation of PeU residues. Upon the formation of a duplex with the target RNA (white strand), the PeU is separated by base pairs, resulting in “light‐up” detection with high sensitivity. This linear probe also discriminated a fully matched RNA strand from a single‐base mismatch. More information can be found in the full paper by H. Asanuma and K. Murayama on page 120 in Issue 1, 2020 (DOI: 10.1002/cbic.201900498).