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Identification and Characterization of Citrus Peel Uronic Acid Oxidase
Author(s) -
Wei Yifeng,
Tan Yee Ling,
Ang Ee Lui,
Zhao Huimin
Publication year - 2020
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201900546
Subject(s) - uronic acid , bioconversion , chemistry , pichia pastoris , biochemistry , citrus × sinensis , orange (colour) , food science , recombinant dna , fermentation , polysaccharide , gene
Uronic acid‐rich plant materials such as pectin are potential renewable feedstocks for the chemical industry. Uronic acid oxidase activity was first reported in extracts of citrus leaves, and was subsequently found to be widely distributed in plants, with the highest activity detected in extracts of the pectin‐rich citrus peel. Herein we report the identification of the primary sequence of uronic acid oxidase from extracts of the peel of Citrus sinensis , by partial purification and protein mass spectrometry. Activity of the enzyme, a member of the berberine bridge family, was confirmed by recombinant expression in Pichia pastoris . Biochemical characterization of the recombinant enzyme is reported. Our findings facilitate further investigation of the biological function of uronic acid oxidation in the economically important orange fruit, and also provide a basis for the development of a catalyst for bioconversion of uronic acids.