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19 F NMR Spectroscopy Tagging and Paramagnetic Relaxation Enhancement‐Based Conformation Analysis of Intrinsically Disordered Protein Complexes
Author(s) -
Somlyay Máté,
Ledolter Karin,
Kitzler Manuel,
Sandford Graham,
Cobb Steven L.,
Konrat Robert
Publication year - 2020
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201900453
Subject(s) - intrinsically disordered proteins , nuclear magnetic resonance spectroscopy , chemistry , paramagnetism , two dimensional nuclear magnetic resonance spectroscopy , relaxation (psychology) , crystallography , stereochemistry , biochemistry , biology , physics , quantum mechanics , neuroscience
The combination of 19 F NMR spectroscopy tagging and paramagnetic relaxation enhancement (PRE) NMR spectroscopy experiments was evaluated as a versatile method to probe protein–protein interactions and conformational changes of intrinsically disordered proteins upon complex formation. The feasibility of the approach is illustrated with an application to the Myc‐Max protein complex; this is an oncogenic transcription factor that binds enhancer box DNA fragments. The single cysteine residue of Myc was tagged with highly fluorinated [ 19 F]3,5‐bis(trifluoromethyl)benzyl bromide. Structural dynamics of the protein complex were monitored through intermolecular PREs between 19 F‐Myc and paramagnetic (1‐oxyl‐2,2,5,5‐tetramethyl‐Δ3‐pyrroline‐3‐methyl)methanethiosulfonate (MTSL)‐tagged) Max. The 19 F R 2 relaxation rates obtained with three differently MTSL‐tagged Max mutants revealed novel insights into the differential structural dynamics of Myc‐Max bound to DNA and the tumour suppressor breast cancer antigen 1. Given its ease of implementation, fruitful applications of this strategy to structural biology and inhibitor screening can be envisaged.