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Molecular Structure of Binary Chromium(III)–DNA Adducts
Author(s) -
Brown Silas,
Lockart Molly M.,
Thomas C. Sumner,
Bowman Michael K.,
Woski Stephen A.,
Vincent John B.
Publication year - 2020
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201900436
Subject(s) - chromium , guanine , chemistry , dna , oligonucleotide , adduct , mutagen , chromium compounds , dna adduct , stereochemistry , nucleotide , biochemistry , organic chemistry , gene
Chromium(VI) is a carcinogen and mutagen, and its mechanisms of action are proposed to involve binding of its reduction product, chromium(III), to DNA. The manner in which chromium(III) binds DNA has not been established, particularly at a molecular level. Analysis of oligonucleotide duplex DNAs by NMR, EPR, and IR spectroscopies in the presence of chromium(III) allows the elucidation of the Cr binding site. The metal centers were found to interact exclusively with guanine N7 positions. No evidence of chromium interactions with other bases or backbone phosphates nor of Cr forming intra‐strand crosslinks between neighboring guanine residues was observed.