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Front Cover: Identification of mNeonGreen as a pH‐Dependent, Turn‐On Fluorescent Protein Sensor for Chloride (ChemBioChem 14/2019)
Author(s) -
Tutol Jasmine N.,
Kam Hiu C.,
Dodani Sheel C.
Publication year - 2019
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201900408
Subject(s) - chromophore , fluorescence , chloride , green fluorescent protein , chemistry , fluorescent protein , tyrosine , biophysics , residue (chemistry) , biochemistry , biology , photochemistry , organic chemistry , physics , optics , gene
mNeonGreen: A bright idea for chloride : Chloride‐sensitive fluorescent proteins generated from natural and laboratory‐guided evolution have a characteristic tyrosine residue that interacts with the anion and π‐stacks with the chromophore. Through a structure‐guided approach, we have identified that the fluorescent protein mNeonGreen, based on lan YFP from the cephalochordate Branchiostoma lanceolatum , lacks this interaction but still binds chloride. Here, we show mNeonGreen displayed as the light bulb filament, lighting up in the presence of chloride to generate a pH‐dependent, turn‐on fluorescent response. This discovery sets the stage to further engineer mNeonGreen as a new tool for imaging cellular chloride dynamics. More information can be found in the communication by S. C. Dodani et al. on page 1759 in Issue 14, 2019 (DOI: 10.1002/cbic.201900147).