Premium
Front Cover: Combining Chemical Synthesis and Enzymatic Methylation to Access Short RNAs with Various 5′ Caps (ChemBioChem 13/2019)
Author(s) -
Muthmann Nils,
Guez Théo,
Vasseur JeanJacques,
Jaffrey Samie R.,
Debart Françoise,
Rentmeister Andrea
Publication year - 2019
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201900371
Subject(s) - rna , methylation , messenger rna , methyltransferase , computational biology , chemistry , biology , biochemistry , dna , gene
During a “capping ceremony” , the RNA snake obtains one of three distinct 5′‐caps, changing its identity to either snRNA or mRNA. In our work, we have combined chemical and enzymatic synthesis of RNA with site‐specific methyltransferases (MTases) to obtain highly cap‐modified RNAs on a preparative scale. MTases hTgs and GlaTgs allow access to snRNAs that are either mono‐ or dimethylated at the N2 position of the terminal G (crowns 2m,7mGppp and 2m,2m,7mGppp, left and center), whereas CMTR1 yielded the cap1 structure (right crown), a structural motif in mRNA important for translation. More information can be found in the full paper by F. Debart. A. Rentmeister, et al. on page 1693 in Issue 13, 2019 (DOI: 10.1002/cbic.201900037). Photo of Montpellier Cathedral by Wolfgang Staudt, protected under a Creative Commons License. Copyright for the image: Nina Knubel and Dr. Daniela Stummer.