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Transaminase‐Catalyzed Continuous Synthesis of Biogenic Aldehydes
Author(s) -
Contente Martina L.,
Paradisi Francesca
Publication year - 2019
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201900356
Subject(s) - transaminase , cofactor , reagent , catalysis , pyridoxal 5 phosphate , chemistry , pyridoxal , biochemistry , pyridoxal phosphate , combinatorial chemistry , enzyme , organic chemistry
The physiological role of biogenic aldehydes, such as 3,4‐dihydroxyphenylacetaldehyde (DOPAL), has been associated with cardiovascular and neurodegenerative disorders. The availability of these substrates is limited and robust synthetic methodologies would greatly facilitate further biological studies. Herein, a transaminase‐mediated single‐step process in continuous mode, which leads to excellent product yields (90–95 %), is reported. Coimmobilization of the pyridoxal phosphate (PLP) cofactor eliminated the need for exogenous addition of this reagent without affecting the longevity of the system, delivering a truly self‐sufficient process.