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In‐Cell EPR: Progress towards Structural Studies Inside Cells
Author(s) -
Bonucci Alessio,
Ouari Olivier,
Guigliarelli Bruno,
Belle Valérie,
Mileo Elisabetta
Publication year - 2020
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201900291
Subject(s) - electron paramagnetic resonance , site directed spin labeling , biomolecule , context (archaeology) , electron paramagnetic resonance spectroscopy , intracellular , nanotechnology , biophysics , structural biology , chemistry , computational biology , materials science , nuclear magnetic resonance , biology , biochemistry , physics , paleontology
Exploring the structure and dynamics of biomolecules in the context of their intracellular environment has become the ultimate challenge for structural biology. As the cellular environment is barely reproducible in vitro, investigation of biomolecules directly inside cells has attracted a growing interest. Among magnetic resonance approaches, site‐directed spin labeling (SDSL) coupled to electron paramagnetic resonance (EPR) spectroscopy provides competitive and advantageous features to capture protein structure and dynamics inside cells. To date, several in‐cell EPR approaches have been successfully applied to both bacterial and eukaryotic cells. In this review, the major advances of in‐cell EPR spectroscopy are summarized, as well as the challenges this approach still poses.