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Targeted Heating of Enzyme Systems Based on Photothermal Materials
Author(s) -
Wang Xuerui,
Ou Gang,
Zhou Ke,
Wang Xiangqing,
Wang Licheng,
Zhang Xiaoyan,
Feng Yi,
Bai Yunpeng,
Wu Hui,
Xu Zhiping,
Ge Jun
Publication year - 2019
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201900267
Subject(s) - enzyme , chemistry , catalysis , enzyme catalysis , lipase , molecule , immobilized enzyme , photothermal therapy , combinatorial chemistry , reaction rate , glucose oxidase , covalent bond , substrate (aquarium) , degradation (telecommunications) , chemical engineering , materials science , organic chemistry , nanotechnology , oceanography , geology , engineering , telecommunications , computer science
Abstract This study demonstrates that the enzymatic reaction rate can be increased significantly by targeted heating of the microenvironment around the enzyme, while maintaining the reaction system at environmental temperature. Enzyme molecules are covalently attached to the surface of Fe 3 O 4 @reduced graphite oxide (rGO). Under visible‐light irradiation, the reaction rate catalyzed by the enzyme–Fe 3 O 4 @rGO system is clearly enhanced relative to that of the free enzyme and a mixture of free enzyme and Fe 3 O 4 @rGO. This local heating mechanism contributes to promotion of the enzymatic reactions of the targeted heating of the enzyme (THE) system, which has been validated by using different enzymes, including lipase, glucose oxidase, and organophosphorus hydrolase. These results indicate that targeted heating of the catalytic centers has the same effect on speeding up reactions as that of traditional heating methods, which treat the whole reaction system. As an example, it is shown that the THE system promotes the sensitivity of an enzyme screen‐printed electrode by 14 times at room temperature, which implies that the THE system can be advantageous in improving enzyme efficiency, especially if heating the entire system is impossible or could lead to degradation of substrates or damage of components, such as in vitro bioanalysis of frangible molecules or in vivo diagnosis.