Premium
Green‐Emitting Rhodamine Dyes for Vital Labeling of Cell Organelles Using STED Super‐Resolution Microscopy
Author(s) -
Grimm Florian,
Nizamov Shamil,
Belov Vladimir N.
Publication year - 2019
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201900177
Subject(s) - sted microscopy , organelle , fluorescence , microscopy , rhodamine , confocal microscopy , confocal , biophysics , super resolution microscopy , fluorescence microscope , chemistry , live cell imaging , stimulated emission , microbiology and biotechnology , cell , biochemistry , biology , laser , optics , physics
Fluorescence microscopy reveals the localization, spatial distribution, and temporal dynamics of the specifically labeled organelles in living cells. Labeling with exogenous conjugates prepared from fluorescent dyes and small molecules (ligands) is an attractive alternative to the use of fluorescent proteins, but proved to be challenging due to insufficient cell‐permeability of the probes, unspecific staining, or low dye brightness. We evaluated four green‐emitting rhodamine dyes and their conjugates intended for the specific labeling of lysosomes, mitochondria, tubulin, and actin in living cells. The imaging performance of the probes in living human fibroblasts has been studied by using confocal and stimulated emission depletion (STED) super‐resolution microscopy with a commercial 595 nm STED laser. Two bright and photostable dyes ( LIVE 510 and LIVE 515 ) provide specific and versatile staining.