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Front Cover: Fluorescing Isofunctional Ribonucleosides: Assessing Adenosine Deaminase Activity and Inhibition (ChemBioChem 5/2019)
Author(s) -
Ludford Paul T.,
Rovira Alexander R.,
Fin Andrea,
Tor Yitzhak
Publication year - 2019
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201900080
Subject(s) - adenosine deaminase , deamination , inosine , adenosine , front cover , cover (algebra) , chemistry , pyrimidine , combinatorial chemistry , biochemistry , enzyme , mechanical engineering , engineering
The front cover picture shows a fluorescence‐based assay utilizing an emissive adenosine surrogate, which “illuminates” the study of adenosine deaminase (ADA) and the discovery of its inhibitors. tz A, an isothiazolo[4,3‐ d ]pyrimidine‐based adenosine analogue, is effectively deaminated by ADA to yield the corresponding inosine analogue. The distinct photophysics of the emissive analogues opens an optical window into this conversion, which is unattainable by the native non‐emissive counterparts. This facilitates real‐time analyses of deamination reactions in the absence and presence of potential inhibitors. As ADA′s high expression levels are associated with unfavorable prognosis in certain cancers, such assays accelerate the discovery of new inhibitors. More information can be found in the full paper by Y. Tor et al. on page 718 in Issue 5, 2019 (DOI: 10.1002/cbic.201800665).