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A FRET‐Based Near‐Infrared Fluorescent Probe for Ratiometric Detection of Cysteine in Mitochondria
Author(s) -
Xia Shuai,
Zhang Yibin,
Fang Mingxi,
Mikesell Logan,
Steenwinkel Tessa E.,
Wan Shulin,
Phillips Tyler,
Luck Rudy L.,
Werner Thomas,
Liu Haiying
Publication year - 2019
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201900071
Subject(s) - cysteine , fluorescence , förster resonance energy transfer , chemistry , rhodamine , photochemistry , acceptor , coumarin , autofluorescence , hydrogen peroxide , biophysics , in vivo , biochemistry , organic chemistry , enzyme , biology , microbiology and biotechnology , physics , quantum mechanics , condensed matter physics
We report a near‐infrared fluorescent probe A for the ratiometric detection of cysteine based on FRET from a coumarin donor to a near‐infrared rhodamine acceptor. Upon addition of cysteine, the coumarin fluorescence increased dramatically up to 18‐fold and the fluorescence of the rhodamine acceptor decreased moderately by 45 % under excitation of the coumarin unit. Probe A has been used to detect cysteine concentration changes in live cells ratiometrically and to visualize fluctuations in cysteine concentrations induced by oxidation stress through treatment with hydrogen peroxide or lipopolysaccharide (LPS). Finally, probe A was successfully applied for the in vivo imaging of Drosophila melanogaster larvae to measure cysteine concentration changes.