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Artificial Cellulosome Complex from the Self‐Assembly of Ni‐NTA‐Functionalized Polymeric Micelles and Cellulases
Author(s) -
Lu Lin,
Zhang Libo,
Yuan Liang,
Zhu Tianyu,
Chen Wilfred,
Wang Guiren,
Wang Qian
Publication year - 2019
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201900061
Subject(s) - cellulase , micelle , chemistry , amphiphile , copolymer , cellulose , polymerization , polymer chemistry , polymer , combinatorial chemistry , organic chemistry , chemical engineering , aqueous solution , engineering
Polymer–protein core–shell nanoparticles have been explored for enzyme immobilization. This work reports on the development of functional polymeric micelles for immobilizing His 6 ‐tagged cellulases with controlled spatial orientation of enzymes, resulting in “artificial cellulosomes” for effective cellulose hydrolysis. Poly(styrene)‐ b ‐poly(styrene‐ alt ‐maleic anhydride) was prepared through one‐pot reversible addition–fragmentation chain‐transfer polymerization and modified with nitrilotriacetic acid (NTA) to afford an amphiphilic block copolymer. The self‐assembled polymer was mixed with a solution of NiSO 4 to form Ni‐NTA‐functionalized micelles, which could successfully capture His 6 ‐tagged cellulases and form hierarchically structured core–shell nanoparticles with cellulases as the corona. Because the anchored enzymes are site‐specifically oriented and in close proximity, synergistic catalysis that results in over twofold activity enhancement has been achieved.