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Genetically Encoded Libraries of Constrained Peptides
Author(s) -
Bosma Tjibbe,
Rink Rick,
Moosmeier Markus A.,
Moll Gert N.
Publication year - 2019
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201900031
Subject(s) - phage display , proteases , computational biology , peptide library , directed molecular evolution , antimicrobial peptides , peptide , directed evolution , genetically engineered , protein engineering , yeast , biology , biochemistry , chemistry , gene , peptide sequence , enzyme , mutant
Many therapeutic peptides can still be improved with respect to target specificity, target affinity, resistance to peptidases/proteases, physical stability, and capacity to pass through membranes required for oral delivery. Several modifications can improve the peptides’ properties, in particular those that impose (a) conformational constraint(s). Screening of constrained peptides and the identification of hits is greatly facilitated by the generation of genetically encoded libraries. Recent breakthrough bacterial, phage, and yeast display screening systems of ribosomally synthesized post‐translationally constrained peptides, particularly those of lanthipeptides, are earning special attention. Here we provide an overview of display systems for constrained, genetically encoded peptides and indicate prospects of constrained peptide‐displaying phage and bacterial systems as such in vivo.